Question: What Is The Purpose Of Mannitol Salt Agar Quizlet?

What would be the effect of removing the sodium chloride from mannitol salt agar plates?

What would be the likely consequences of omitting the NaCl in Mannitol Salt Agar.


Non-staphylococcus bacteria would be able to grow on the media.

This may lead to false positives for Non-staphylococcus that can ferment mannitol..

Is blood agar selective or differential?

Blood Agar This medium can be made selective by the addition of antibiotics. This medium is differential because: Blood Agar tests for the production of hemolysin which breaks down red blood cells.

What type of bacteria grow on blood agar?

Blood Agar is used to grow a wide range of pathogens particularly those that are more difficult to grow such as Haemophilus influenzae, Streptococcus pneumoniae and Neisseria species. It is also required to detect and differentiate haemolytic bacteria, especially Streptococcus species.

How many types of MSA are there?

MSA is classified by two types: parkinsonian and cerebellar. The type depends on the symptoms you have at diagnosis.

Master Servicers AgreementA Master Servicers Agreement (“MSA”) is contract between two parties with an ongoing project or business relationship that offers a flexible system for completing a project over time when there will be decisions to be made along the way.

Is E coli a mannitol fermenter?

Gram-negative bacteria like E. coli and P. … Staphylococcus aureus is also able to ferment mannitol, because this bacterial species has the enzyme coagulase required for the process.

What ingredient makes MSA differential?

sugar mannitolThe differential ingredient in MSA is the sugar mannitol. Organisms capable of using mannitol as a food source will produce acidic byproducts of fermentation that will lower the pH of the media. The acidity of the media will cause the pH indicator, phenol red, to turn yellow.

What bacteria grow on mannitol salt agar?

Most pathogenic staphylococci, such as Staphylococcus aureus, will ferment mannitol. Most non-pathogenic staphylococci will not ferment mannitol. The Staphylococcus aureus ferments mannitol and turns the medium yellow. The Serratia marcescens does not grow because of the high salt content.

Would removal of sodium chloride from MSA?

Yes, the removal of sodium chloride from MSA will alter the medium’s sensitivity or specificity to a great extent.

What type of media is blood agar?

Blood agar is an enriched, bacterial growth medium. Fastidious organisms, such as streptococci, do not grow well on ordinary growth media. Blood agar is a type of growth medium (trypticase soy agar enriched with 5% sheep blood) that encourages the growth of bacteria, such as streptococci, that otherwise wouldn’t grow.

What is the role of sodium chloride in MSA and how does it work?

Answer and Explanation: Sodium chloride is used in MSA to provide the essential fermentation substrate. It limits the growth of most organisms apart from bacteria in mixed…

How does the NaCl IN MSA kill organisms?

Provides the substrate for fermentation and makes the medium differential. What does the NaCl contained in MSA do? Makes the medium selective because its concentration is high enough to dehydrate and kill most bacteria. … Interoretation: organism is inhibited by NaCl.

What is the function of the 7.5% NaCl in mannitol salt agar?

Mannitol salt agar is selective in allowing bacteria capable of growth in 7.5% NaCl while preventing the growth of salt sensitive microbes. Additionally, mannitol salt agar contains the sugar mannitol.

Why is MSA selective and differential?

MSA is a Selective Medium because of its high (7.5%) sodium chloride concentration that inhibits the growth of most organisms. MSA is a Differential Medium because of the presence of the sugar mannitol and the pH indicator Phenol Red.

Will E coli grow on mannitol salt agar?

(A) Staphylococcus aureus, (B) Staphylococcus epidermidis, and (C) Escherichia coli streaked on a mannitol salt agar plate. … The growth of E. coli was inhibited by the high salt concentration.

Can Streptococcus grow on mannitol salt agar?

Both streptococcal organisms are catalase negative and beta-hemolytic on sheep blood agar plates. Also, neither would grow on the mannitol salt agar. Streptococcus pyogenes is sensitive to growth inhibition by bacitracin, whereas Streptococcus agalactiae (group B streptococci) is not.

What is the purpose of using mannitol salt agar?

Mannitol salt agar or MSA is a commonly used selective and differential growth medium in microbiology. It encourages the growth of a group of certain bacteria while inhibiting the growth of others.

What is the purpose of MSA?

Mannitol Salt Agar (MSA) is used as a selective and differential medium for the isolation and identification of Staphylococcus aureus from clinical and non-clinical specimens. It encourages the growth of a group of certain bacteria while inhibiting the growth of others.